Regulatory
Part:BBa_J119323
Designed by: MWSU Genetics Fall 2013 Tuesday 2:00 lab Group: Eckdahl Lab (2013-11-18)
pTac mutant -32 A
This part was designed and clone by students in the Missouri Western State University Genetics class of Fall 2013. It was cloned into the pClone Green vector (BBa_J119137) using Golden Gate Assembly and synthetic oligonucleotides. The mutation was a transition of the G at position -32 to A. The mutation had a large negative effect on the function of the promoter (see Experience page).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |